Coding

Part:BBa_K1033260

Designed by: Anders Edlund   Group: iGEM13_Uppsala   (2013-09-13)

Toxin-antitoxin system from Lactobacillus plantarum

This is a toxin-antitoxin system (pem) together with a natural putative promotor from the Lactobacillus plantarum NC7 plasmid p256. The main use of the toxin-antitoxin system is to make the plasmid more stabile without having to rely on antibiotic resistance. The antitoxin has a shorter halflife then the antitoxin. If the cell were to lose the plasmid the toxin will remain longer in the cell and thereby kill it. It has been shown to increase segregational stability under non-selective pressure. The system has experimentally been shown to allow 88-100% retention of a plasmid after 80 generations. [1]

[1] Plasmid p256 from Lactobacillus plantarum represents a new type of replicon in lactic acid bacteria, and contains a toxin–antitoxin-like plasmid maintenance system, Microbiology, Elisabeth Sorvig et al. September 30 2004

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 158
    Illegal SpeI site found at 534
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 158
    Illegal SpeI site found at 534
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 158
    Illegal SpeI site found at 534
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 158
    Illegal SpeI site found at 534
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None